Journal: Journal of immunology (Baltimore, Md. : 1950)
Article Title: Characterization of a dipeptide motif regulating IFN-gamma receptor 2 plasma membrane accumulation and IFN-gamma responsiveness.
doi: 10.4049/jimmunol.173.6.3991
Figure Lengend Snippet: FIGURE 7. IFN- responsiveness of IFN-R2-de- ficient fibroblasts transiently transfected with WT and mutant constructs. Cells were stimulated with IFN- (1000 U/ml) for 15 min before harvesting. Represen- tative phospho-STAT1 (P-STAT1) accumulation pat- terns are shown. Densitometry was used to determine the phospho-STAT1/STAT1 ratio. , p 0.005 com- pared with SP/GFP/IFNGR2.
Article Snippet: Polyclonal rabbit-anti-human P-STAT1 (Tyr701) Ab (Cell Signaling, Beverly, MA) was used at a 1/1000 dilution.
Techniques: Transfection, Mutagenesis, Construct